MAP2K1 ELISA Kit for Human(Homo sapiens) | EIAab.comMAP2K1 ELISA Kit for Human(Homo sapiens)

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MAP2K1 (Gene name),

Dual specificity mitogen-activated protein kinase kinase 1 (Protein name ), MP2K1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

MAP2K1(MEK1;PRKMK1);

Protein name:

Dual specificity mitogen-activated protein kinase kinase 1(MAP kinase kinase 1;MAPKK 1;MKK1);

Alternative:

MAPK/ERK kinase 1(MEK 1);ERK activator kinase 1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with MORG1. Interacts with ARRB2. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts with Yersinia yopJ. Interacts with isoform 1 of VRK2.

Function:

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

393

Sequence:

50:

100:

150:

200:

250:

300:

350:

393:

DLKQLMVHAF | IKRSDAEEVD | FAGWLCSTIG | LNQPSTPTHA | AGV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000302486

UniGene:

Hs.145442

MIM:

176872

MIM:

115150

Pfam:

PF00069

String:

Q02750

KEGG:

hsa:5604

SMR:

Q02750

Uniprot:

Q02750

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ELISA Kit for Human MAP kinase kinase 1

Cat.:

E10995h

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96T

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ELISA Kit for Human MAP kinase kinase 1

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E10995m

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96T

ELISA Kit for Human MAP kinase kinase 1

Cat.:

E10995r

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96T

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ELISA Kit for Human MAP kinase kinase 1

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CLIA Kit for Human MAP kinase kinase 1

Cat.:

U10995m

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Packing:

96T

CLIA Kit for Human MAP kinase kinase 1

Cat.:

U10995h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Human MAP kinase kinase 1

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CLIA Kit for Human MAP kinase kinase 1

Cat.:

U10995r

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96T

Polyclonal Antibody for Human MAP kinase kinase 1

Cat.:

P10995Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human MAP kinase kinase 1

Monoclonal Antibody for Human MAP kinase kinase 1

Protein for Human MAP kinase kinase 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases."
Seger R. , Seger D. , Lozeman F.J. , Ahn N.G. , Graves L.M. , Campbell J.S. , Ericsson L. , Harrylock M. , Jensen A.M. , Krebs E.G.
J. Biol. Chem.267:25628-25631(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);PARTIAL PROTEIN SEQUENCE;TISSUE SPECIFICITY
tissue: T-cell.
2.

"Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2."
Zheng C.-F. , Guan K.-L.
J. Biol. Chem.268:11435-11439(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
3.

"Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues."
Zheng C.-F. , Guan K.-L.
EMBO J.13:1123-1131(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-218 AND SER-222;MUTAGENESIS
4.

"Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor."
Duesbery N.S. , Webb C.P. , Leppla S.H. , Gordon V.M. , Klimpel K.R. , Copeland T.D. , Ahn N.G. , Oskarsson M.K. , Fukasawa K. , Paull K.D. , Vande Woude G.F.
Science280:734-737(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CLEAVAGE BY ANTHRAX LETHAL FACTOR;PROTEIN SEQUENCE OF 9-17
5.

"Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor."
Vitale G. , Bernardi L. , Napolitani G. , Mock M. , Montecucco C.
Biochem. J.352:739-745(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CLEAVAGE BY ANTHRAX LETHAL FACTOR
6.

"The MAP kinase pathway is required for entry into mitosis and cell survival."
Liu X. , Yan S. , Zhou T. , Terada Y. , Erikson R.L.
Oncogene23:763-776(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;FUNCTION
7.

"Role of group A p21-activated kinases in activation of extracellular-regulated kinase by growth factors."
Beeser A. , Jaffer Z.M. , Hofmann C. , Chernoff J.
J. Biol. Chem.280:36609-36615(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-298
8.

"Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation."
Mukherjee S. , Keitany G. , Li Y. , Wang Y. , Ball H.L. , Goldsmith E.J. , Orth K.
Science312:1211-1214(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH YOPJ;ACETYLATION
9.

"Interaction with MEK causes nuclear export and downregulation of peroxisome proliferator-activated receptor gamma."
Burgermeister E. , Chuderland D. , Hanoch T. , Meyer M. , Liscovitch M. , Seger R.
Mol. Cell. Biol.27:803-817(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH PPARG
10.

"Final stages of cytokinesis and midbody ring formation are controlled by BRUCE."
Pohl C. , Jentsch S.
Cell132:832-845(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BIRC6/BRUCE
11.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-286;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
12.

"Protein kinase SGK1 enhances MEK/ERK complex formation through the phosphorylation of ERK2: implication for the positive regulatory role of SGK1 on the ERK function during liver regeneration."
Won M. , Park K.A. , Byun H.S. , Kim Y.R. , Choi B.L. , Hong J.H. , Park J. , Seok J.H. , Lee Y.H. , Cho C.H. , Song I.S. , Kim Y.K. , Shen H.M. , Hur G.M.
J. Hepatol.51:67-76(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SGK1
13.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
14.

"VRK2 inhibits mitogen-activated protein kinase signaling and inversely correlates with ErbB2 in human breast cancer."
Fernandez I.F. , Blanco S. , Lozano J. , Lazo P.A.
Mol. Cell. Biol.30:4687-4697(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH VRK2
15.

"Signaling by dual specificity kinases."
Dhanasekaran N. , Premkumar Reddy E.
Oncogene17:1447-1455(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
16.

"The RAF proteins take centre stage."
Wellbrock C. , Karasarides M. , Marais R.
Nat. Rev. Mol. Cell Biol.5:875-885(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON ENZYME REGULATION
17.

"The ERK signaling cascade--views from different subcellular compartments."
Yao Z. , Seger R.
BioFactors35:407-416(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
18.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"The ERK cascade: distinct functions within various subcellular organelles."
Wortzel I. , Seger R.
Genes Cancer2:195-209(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
20.

"Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition."
Ohren J.F. , Chen H. , Pavlovsky A. , Whitehead C. , Zhang E. , Kuffa P. , Yan C. , McConnell P. , Spessard C. , Banotai C. , Mueller W.T. , Delaney A. , Omer C. , Sebolt-Leopold J. , Dudley D.T. , Leung I.K. , Flamme C. , Warmus J. , more...
Nat. Struct. Mol. Biol.11:1192-1197(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 62-392 IN COMPLEX WITH ATP AND INHIBITOR
21.

"4-anilino-5-carboxamido-2-pyridone derivatives as noncompetitive inhibitors of mitogen-activated protein kinase kinase."
Spicer J.A. , Rewcastle G.W. , Kaufman M.D. , Black S.L. , Plummer M.S. , Denny W.A. , Quin J. III , Shahripour A.B. , Barrett S.D. , Whitehead C.E. , Milbank J.B. , Ohren J.F. , Gowan R.C. , Omer C. , Camp H.S. , Esmaeil N. , Moore K. , Sebolt-Leopold J.S. , more...
J. Med. Chem.50:5090-5102(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 62-393 IN COMPLEX WITH ATP AND INHIBITOR
22.

"2-Alkylamino- and alkoxy-substituted 2-amino-1,3,4-oxadiazoles-O-Alkyl benzohydroxamate esters replacements retain the desired inhibition and selectivity against MEK (MAP ERK kinase)."
Warmus J.S. , Flamme C. , Zhang L.Y. , Barrett S. , Bridges A. , Chen H. , Gowan R. , Kaufman M. , Sebolt-Leopold J. , Leopold W. , Merriman R. , Ohren J. , Pavlovsky A. , Przybranowski S. , Tecle H. , Valik H. , Whitehead C. , Zhang E.
Bioorg. Med. Chem. Lett.18:6171-6174(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.62 ANGSTROMS) OF 62-393 IN COMPLEX WITH ATP AND INHIBITOR
23.

"Crystal structures of MEK1 binary and ternary complexes with nucleotides and inhibitors."
Fischmann T.O. , Smith C.K. , Mayhood T.W. , Myers J.E. , Reichert P. , Mannarino A. , Carr D. , Zhu H. , Wong J. , Yang R.S. , Le H.V. , Madison V.S.
Biochemistry48:2661-2674(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 35-393 IN COMPLEX WITH ADP AND INHIBITOR
24.

"Beyond the MEK-pocket: can current MEK kinase inhibitors be utilized to synthesize novel type III NCKIs? Does the MEK-pocket exist in kinases other than MEK?"
Tecle H. , Shao J. , Li Y. , Kothe M. , Kazmirski S. , Penzotti J. , Ding Y.H. , Ohren J. , Moshinsky D. , Coli R. , Jhawar N. , Bora E. , Jacques-O'Hagan S. , Wu J.
Bioorg. Med. Chem. Lett.19:226-229(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 62-382 IN COMPLEX WITH ATP AND INHIBITOR
25.

"RDEA119/BAY 869766: a potent, selective, allosteric inhibitor of MEK1/2 for the treatment of cancer."
Iverson C. , Larson G. , Lai C. , Yeh L.T. , Dadson C. , Weingarten P. , Appleby T. , Vo T. , Maderna A. , Vernier J.M. , Hamatake R. , Miner J.N. , Quart B.
Cancer Res.69:6839-6847(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 62-393 IN COMPLEX WITH ATP AND INHIBITOR
26.

"Structure-based design and synthesis of pyrrole derivatives as MEK inhibitors."
Wallace M.B. , Adams M.E. , Kanouni T. , Mol C.D. , Dougan D.R. , Feher V.A. , O'Connell S.M. , Shi L. , Halkowycz P. , Dong Q.
Bioorg. Med. Chem. Lett.20:4156-4158(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 62-382 IN COMPLEX WITH ADP AND INHIBITOR
27.

"Discovery of TAK-733, a potent and selective MEK allosteric site inhibitor for the treatment of cancer."
Dong Q. , Dougan D.R. , Gong X. , Halkowycz P. , Jin B. , Kanouni T. , O'Connell S.M. , Scorah N. , Shi L. , Wallace M.B. , Zhou F.
Bioorg. Med. Chem. Lett.21:1315-1319(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 62-382 IN COMPLEX WITH ATP AND INHIBITOR
28.

"Design and synthesis of novel allosteric MEK inhibitor CH4987655 as an orally available anticancer agent."
Isshiki Y. , Kohchi Y. , Iikura H. , Matsubara Y. , Asoh K. , Murata T. , Kohchi M. , Mizuguchi E. , Tsujii S. , Hattori K. , Miura T. , Yoshimura Y. , Aida S. , Miwa M. , Saitoh R. , Murao N. , Okabe H. , Belunis C. , more...
Bioorg. Med. Chem. Lett.21:1795-1801(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 62-393
29.

"Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome."
Rodriguez-Viciana P. , Tetsu O. , Tidyman W.E. , Estep A.L. , Conger B.A. , Cruz M.S. , McCormick F. , Rauen K.A.
Science311:1287-1290(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CFC3 SER-53 AND CYS-130
30.

"Mutation and phenotypic spectrum in patients with cardio-facio-cutaneous and Costello syndrome."
Schulz A.L. , Albrecht B. , Arici C. , van der Burgt I. , Buske A. , Gillessen-Kaesbach G. , Heller R. , Horn D. , Hubner C.A. , Korenke G.C. , Konig R. , Kress W. , Kruger G. , Meinecke P. , Mucke J. , Plecko B. , Rossier E. , Schinzel A. , more...
Clin. Genet.73:62-70(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CFC3 VAL-128

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